Manipulation in Documentaries free essay sample

Every night when we sit down to watch our much loved Grey’s Anatomy or Desperate Housewives, we know for a fact they are fictional dramas which allow us to escape the reality and boredom of our lives. We also realise the scripted shows use many tactics to manipulate our thinking. The producer does this to position us towards a specific view of life. But what do we expect from a documentary? We know documentaries to be faithful recounts of events; however, these events may be subject to just as much manipulation as the intriguing plots in our addictive shows. Many documentary makers include their individual cultural assumptions and discourses to distort the truth and position the target audience to react in a desired manner. This can be seen in the documentary, ‘Supersize Me’ by Morgan Spurlock and ‘Great White – Deep Trouble’ showcasing Peter Benchley, David Doubilet and Rodney Fox. Both producers desire to position the audience towards their discourse through their documentary. We will write a custom essay sample on Manipulation in Documentaries or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page They accomplish this by presenting snippets of films, opinions and facts that agree with their discourse and disposing of the rest. ‘Super Size Me’, written, produced, directed by and starring Morgan Spurlock, is a producer dominated documentary. It shows Spurlock endeavouring on a thirty day McDonald’s binge to prove that regular consumption of the food is a fast way to heart disease, high cholesterol and obesity. Spurlock aims to prove his nutritional discourse by taking tests before and after the experiment to show the affects. Before the experiment began, Spurlock’s body showed healthy results both physically and emotionally, however, after the experiment, his body was physically rundown sporting clogged arteries, high cholesterol, weight gain and emotionally he showed early stages of depression. Spurlock’s aim in the documentary is to inform his audience that regular fast food consumption is extremely harmful to your body. It uses both fully narrated and self reflexive types of documentary to present the message. This means voice overs are used to interpret graphs, pictures and facts presented in the documentary to prove that fast food has a significant impact on the nation’s health. He also includes many techniques including camera angles, music and sound, lighting and many elements typical of documentaries. Spurlock uses Mise-en-scene to prove that obesity is a result of extreme fast food intake by purposely filming obese people walking through a shot at McDonalds. Spurlock also uses the technique of interviewing doctors and scientists to grab our attention and make it difficult for us, the audience, to make our own decision. He does this as the majority of us believe scientists and doctors have the most knowledge on this argument and therefore we will agree with his discourse. Similarly the documentary, ‘Great White – Deep Trouble’, produced by John Bredar, attempts to influence the audience to feel sympathy and love for the Great White which to many represents a killer beast. The documentary aims to manipulate us towards the view that sharks are gentle fish that are simply misunderstood by humans. Through the journey of David Doubilet, a famous photographer, the audience are presented with pictures, journal entries and filming of the Great White in the hope of capturing the perfect shot. One of the crew on the mission is Rodney Fox who previously had a bloody encounter with a Great White and survived. Although the majority of us would be too afraid to return to the water, Fox has chosen to partake in the mission of defending the creature. The producer uses this to his advantage by demonstrating that even someone who was attacked by a killing machine managed to love the animal and therefore we should too. The documentary uses techniques in order to create a calm ambience and make the shark seem vulnerable. They use relaxing music and bright lighting to create a sympathetic and almost angelic view of the shark. Camera shots are taken under water allowing the audience to see the scenery and true characteristics of the shark. The documentary provides close up shots to shows the sharks facial features and emotions. It also uses long shots to show the entire body and the provide insight into the sharks natural habitat. The documentary uses the feature of exposition to expose the true qualities of the shark in order to manipulate the audience. Both documentaries possess intertextualities that we are all familiar with. In general, when we see Ronald McDonald we are automatically reminded of McDonalds and their food products. In the Documentary, Spurlock shows Ronald McDonald as an evil clown rather than happy. This represents his negativity towards the restaurant. When watching ‘Great White – Deep Trouble’ our intertextualities towards sharks is that they are vicious man-eating machines like the shark off the movie ‘Jaws’. Although many facts, graphs and discourses are exposed and explained throughout these two documentaries it does not necessarily mean the producer’s opinion is correct. They simply want to manipulate and position the audience to believe what they say in order to sell their view to the public. When we hear the word,’ documentary’ our cultural beliefs tell us they are filled with true facts or recounts of events, however, we need to realise that documentaries are filled with individual discourses which aren’t essentially accurate. We as an audience need to ensure we do not fall victim to the producer’s manipulative techniques that are incorporated in the documentaries. In order to do so we need to be strong in our personal beliefs and discourses. That does not mean you should seclude all opinions from others. Be open to new ideas, but be strong in your own beliefs. That way you will not be manipulated as easily. Make your own decision and don’t let the producer sway that conclusion.

Ethical Considerations in Respect of Advertising, Sales Promotion, Pricing, Product Packaging and Obsolescence Essay Example

Ethical Considerations in Respect of Advertising, Sales Promotion, Pricing, Product Packaging and Obsolescence Essay Example Ethical Considerations in Respect of Advertising, Sales Promotion, Pricing, Product Packaging and Obsolescence Paper Ethical Considerations in Respect of Advertising, Sales Promotion, Pricing, Product Packaging and Obsolescence Paper Assignment: Ethical considerations in respect of advertising, sales promotion, pricing, product packaging and obsolescence. 2011 Introduction Ethics is concerned with what is right and what is wrong. Ethics relate to moral evaluations of decisions and actions as right or wrong on the basis of commonly accepted principles of behaviour (Dibb et. al. , 1997), in other words, ethics are the moral principles and values that govern the actions or/and decisions of an individual or group. They serve as guidelines on how to act rightly and justly when faced with moral dilemmas. Sometimes the line between what is considered ethical and unethical is difficult to distinguish since what is right and wrong differs depending on such factors as nationality, culture, sex etc. Ethics is individually defined and may vary from one person to another. Many people wrongly assume that only actions that violate laws are considered unethical. Some activities can be unethical even though no laws are violated, for example, it can be considered as unethical activity for companies to aggressively promote unhealthy food to children though such promotional practices are generally in the world not viewed as illegal. It is believed that good marketing is ethical marketing. Practicing ethics in marketing means deliberately applying standards of fairness, or moral rights and wrongs, to marketing decision making, behaviour, and practice in the organization. While the most basic ethical principles have been codified as laws and regulations to conform to the standards of society, marketing ethics goes beyond legal and regulatory issues. Ethical marketing practices and principles are the main that establish trust, which will help to gain and maintain the reputation of the company and to build long-term marketing relationships. The purpose of this assignment is to define and explain ethical marketing considerations in respect of advertising, sales promotions, product quality, packaging, obsolescence, pricing and consumer manipulation. Promotion and advertising Some marketing activities may create ethical issues, for example, false, misleading and negative advertising, manipulative or deceptive sales promotions etc. An ethical issue is an identifiable problem, situation or opportunity requiring an individual or organisation to choose from among several actions that must be evaluated as right or wrong, ethical or unethical. Any time any marketing activity causes customers to feel deceived, manipulated or cheated, a marketing ethical issue exists, regardless on the legality of that activity (Dibb et. al. , 1997). The visibility of advertising, coupled with its role as persuasive communication, results in it being the area of marketing most affected by ethical issues. The major ethical issues in advertising and sales promotion are their misleading or deceptive aspects and the social harm attributed to advertising. Unethical actions in advertising can destroy the trust that customers have in the company. First to mention is false, deceptive or misleading advertising. As advertising has the potential to persuade people into commercial transactions that they might otherwise avoid, many governments around the world use legal regulations to control false, deceptive or misleading advertising. An advertisement is deceptive if there is a representation, omission or practice that is likely to mislead consumers in a material way – and is therefore ethically wrong. Advertisers must be able to substantiate claims about product performance. Second to mention is advertising that involves ambiguous statement – statements using words so weak that the viewer, reader or listener must infer advertisers’ intended messages (Dibb et. al. , 1997). Third to mention is advertising that involves violence, profanity and propaganda of sex. Fourth to mention is attack advertisements that unfairly denigrate competitors, so called negative advertising. In negative advertising, the advertiser highlights the disadvantages of competitor products rather than the advantages of their own. Sales promotion Personal selling as a type of sales promotions is to be mentioned regarding ethical issues, because some consumers may perceive salespeople as an unethical when persuading and pressing to purchase the products that they neither need nor want, so called high-pressure selling. A common problem in personal selling is judging about what types of sales activities are acceptable and what are not. Although most sales people are ethical, some do engage in questionable actions, such as aggressive and manipulative tactics, or not telling the customers the truth about the product, or making customers to believe that they will get more value than they actually do. Deceptive or misleading promotion â€Å"includes practices such as overstating the products’ features or performance, luring the customers to the store for a bargain that is out of stock, or running rigged contests† (Kotler et. l. , 2002). Pricing Ethical pricing means such type of pricing so as to avoid taking undue advantage of the highly price-inelastic demand for a product (Schoell, 1990). Price fixing (bid rigging), predatory pricing (dumping), failure to disclose the full price associated with a purchase, price discrimination, price skimming, price cartels and price wars are typical ethical issues in pricing policies. Most of the pricing policies mentioned above are illegal. The emotional and subjective nature of price creates many situations in which misunderstandings between the seller and buyer cause ethical problems. Marketers have the right to price their products so that they earn a reasonable profit, but ethical issues may crop up when company seeks to ear high profits at the expense of its customers. (Dibb et. al. , 1997). Product packaging Deceptive packaging includes exaggerating package contents through subtle design, not filling the package to the top, using misleading labelling, or describing size in the misleading terms (Kotler et. l. , 2002). Product obsolescence Product obsolescence can be planned and functional, planned obsolescence means a strategy of causing products to become obsolete before they actually need replacement (for example, electronics, fashion and computer industry) (Kotler et. al. , 2002), but functional obsolescence occurs when technological breakthroughs render an existing product out-of-date. Three types of planned obsolescence exist: postponed, internationally designed and fashion or style. Postponed obsolescence means holding back and adding product improvements until present inventories run out or demand falls out sharply. Internationally designed obsolescence involves designing a product, or a critical part, to wear out within a given period of time. Fashion or style obsolescence is psychological – new model cars make last year’s models obsolete (Schoell, 1990). References: Dibb, S. , Simkin, L. , Pride, W. M. , Ferrell, O. C. (1997), Marketing, Concepts and strategies, Third European edition, Houghton Mifflin Company, U. S. A, pp. 738-49. Kotler, P. , Armstrong, G. , Saunders, J. , Wong, V. (2002), Principles of marketing, Third European edition, Pearson Education Limited, Edinburgh, England, pp. 43-68. Schoell, W. F. , Guiltinan, J. P. (1990), Marketing contemporary concepts and practices, Fourth edition, Allyn anb Bacon, Massachusetts, pp. 27-29, 60-64, 313, 649.

How would you suggest this should be done Are any of these subsystems Assignment

How would you suggest this should be done Are any of these subsystems more or less important than any others - Assignment Example For example, it will be impossible to attain the mission of an organization without having a clear vision and/ or objective. Examples of subsystems within organizations include goals and values subsystem, human resources subsystems, information and intelligence subsystem, technical subsystem, managerial subsystem and structural subsystem. According to Baskerville and Spagnoletti (2013), the continual interaction between various subsystems creates a pattern that specify what the main system looks like and therefore, it is impossible to gauge the main organizational system by over passing a particular subsystem since the main system relies on interdependent sub-systems. Therefore, when trying to evaluate the strengths and weaknesses of an organization it is a must for all the subsystems to be considered since an organizational is a functional unit that can only operate smoothly with the co-operation and co-ordination of various subsystems which are interdependent to each other. This is to mean that no sub-system is more or less important than the other since an organization cannot operate without a single subsystem missing and hence the evaluation should consider the strengths and weakness of each subsystem in the

Staffing Plan Paper Essay Example | Topics and Well Written Essays - 1000 words

Staffing Plan Paper - Essay Example Developing a strategic plan for staffing in a transport or distribution company is one thing that helps many it in making the right choice of what best they want for themselves (Bechet, 2008). In this step, a human resource manager or the person in charge of this activity has to collect information basing on the kind of service provided by the current employees. This information gathered is very important for companies because they help when enforcing some training and also in the case when the company promotes its employees. After getting the information needed about the available staff members, one can use it in finding out the average input provided by each employee in accordance to the number of hours (Bechet, 2008). An example can be drawn from a transportation company which depends on the number of trips made by drivers in determining ones capability. Most of the companies around the world do have some of the seasons that they consider much busy while other seasons are not taken as such. During the busiest seasons, one can use the statistics in determining the kind of need the company requires for the purpose of making the best from what they have. For instance the transportation company has to have more vehicles during the busiest seasons because this is a time when a company’s products are very much competed in the market. With more vehicles for the purpose of transportation means that the available products will get to the market on time, improving the company’s supply. The fourth step in the staffing plan is determining the number of employees the company requires in terms of drivers responsible for the transportation activity. This must comply with both the busiest seasons and the less busy ones. An additional number of vehicles mean that more drivers have to be employed by the company while having less vehicle leads to the current employees or drivers being overworked

Is Nuclear power our gate to the future (with nuclear or against ) Essay

Is Nuclear power our gate to the future (with nuclear or against ) - Essay Example The information comes from reputable sources such as the U.S. Nuclear Regulatory Commission, U.S. Department of Energy, Energy Information Administration, and U.S. Atomic Energy Commission, Office of Planning and Analysis. The source has no bias because it presents a logical argument about a beneficial energy source that is expensive. I have chosen this source because it compares the cost of nuclear energy and other alternative energy sources. The author mentions ways for reducing the cost of construction of nuclear plants to make it affordable in the society. The main topic of the article is improving efficiency in nuclear power plants. It discusses innovative techniques and safety measures that can ensure nuclear energy plants maintain efficiency. The author supports nuclear power. He chooses this position because he believes that there are innovative measures that can ensure safety in nuclear power plants. Their arguments are based on latest research on technology that is useful in nuclear power plants. The source is credible because it discusses incremental innovations in the digital world, and enhancements of safety margins in an existing power plant. I have chosen this source because it provides a solution for risks involved in nuclear power plants. The dangers and effects of nuclear plants remain the greatest challenge that opponents of nuclear energy discuss. The main topic of the article is making nuclear energy sustainable. The paper analyzes and reviews the challenges that nuclear power must get through before the society accepts it as a sustainable energy source. Pearce is obviously against nuclear power as an energy source because it is not sustainable. He chose this position because of its associated environmental burdens, specifically greenhouse gas emissions and nuclear and radioactive waste disposal. In addition, he cites nuclear insecurity as a danger of nuclear energy,

RP-HPLC-UV Method for Simultaneous Estimation of Ceftriaxone

RP-HPLC-UV Method for Simultaneous Estimation of Ceftriaxone A Validated RP-HPLC-UV method for Simultaneous estimation of Ceftriaxone and Sulbactum in Rat Plasma ABSTRACT: A reverse phase-liquid chromatographic method with UV detection is developed for simultaneous estimation of ceftriaxone sodium and sulbactam sodium in rat plasma. Drugs were extracted from blank plasma by simple protein precipitation technique. Chromatographic separation of these two drugs was done on Phenomenex C18 column (250mm X 4.6mm, i.d, 5ÃŽ ¼m) by using mobile phase consisting of 10mM potassium dihydrogen orthophosphate buffer (pH- 5) and acetonitrile (90:10 % v/v). The developed RP-HPLC method had the acceptable symmetrical peaks good resolution and drugs were eluted with good retention time. The developed bio-analytical method was Linear, precise, and accurate with the concentration range of 20-150 ÃŽ ¼g mL-1 for ceftriaxone and 10-75 ÃŽ ¼g mL-1 for sulbactam. From the developed method we can moniter ceftriaxone and sulbactam sodium concentrations in rat plasma. Keywords: Ceftriaxone sodium, Sulbactam sodium, Liquid chromatography, Rat plasma INTRODUCTION Ceftriaxone[1] (CFX) is a third generation cephalosporin. Chemically it is (6R,7R)-7-{2-(2-amino-4-thiazolyl)-(Z)-2- [methoxyiminuteo-acetamido]-3{[(2,5-dihydro-6-hydroxy-2-methyl-5-oxo-as-triazin-3-yl)thio]methyl}-8-oxo-5-thia-l-azobicyclo [4,2,0] oct-2-ene-2-carboxylic acid. Sulbactam (SBM) chemically (2S,5R)-3,3-Dimethyl-7-oxo-4-thia-1- azabicyclo[3.2.0] heptane -2-carboxylic acid 4,4-dioxide is used as a beta-lactamase inhibitor. Structural formulae of CFX and SBM are given in Fig.1. These drugs are frequently associated in pharmaceutical formulations against meningitis, typhoid, gonorrhoea and urinary tract infections [2]. Sulbactomax is a commercially available pharmaceutical product containing SBM and CFX. The product is available as a dry powder for injection. The product is supplied in different strengths (250 mg+125 mg, 500mg+250 mg, 1gm+0.5gm, 2gm+1gm) of CFX and SBM respectively. Fig.1.Chemical structure of CFX and SBM Sulbactomax is a synergistic antimicrobial mixture with clear in vitro antibacterial activity against a wide spectrum of organisms. SBM not only increases the antibacterial activity of CFX but also shows a moderate antibacterial activity by forming a protein complex with beta-lactamas by irreversibly blockin their destructive hydrolytic activity. Thus, SBM increases the spectrum of activity of CFX. This SBM also binds with some penicillin binding proteins, sensitive strains are often considered more susceptible to the Sulbactomax than CFX alone. In bacterial strains that produce either low amounts of beta lactamase, or none at all, a synergistic effect is witnessed when SBM is associated with CFX that has a complementary affinity for the target sites. Sulbactomax has good active against all the microorganisms which are sensitive/resistant to CFX. Further, it also demonstrates synergistic activity (decrease in minimum inhibitory concentrations for the combination versus those of each component) in a variety of organisms. So it has improved efficacy as compared to CFX alone, lesser side effects, wider spectrum coverage and better results of bacterial MIC (minimum inhibitory concentration) makes this product unique in the world. A literature survey revealed a spectrophotometric [3], spectrofluorimetric in human plasma [4], HPLC for the estimation of marketed formulations [5,6], in human plasma [7] and for the determination of pharmacokinetics in dogs [8], capillary electrophoresis [9] and GC-MS [10] methods for the estimation of CFX and SBM individually and in combined forms. However, from the literature survey there was no method development reported for the simultaneous estimation of CFX and SBM by HPLC in rat plasma. The present communication describes an isocratic liquid chromatography (LC) method for simultaneous determination of CFX sodium and SBM, which can be used for the quality control of the formulation developed and other biological applications. Experimental Chemicals and Reagents All chemicals and reagents used were of analytical grade only. Milli-Q-water was used throughout the process and acetonitrile of HPLC grade were procured from Merck Chemical Laboratories, Bangalore, India. Commercial formulation, CetriaxS injection containing ceftriaxone sodium 1gm and sulbactam sodium 0.5 gm were obtained from the local market. Blank rat plasma was obtained from JSS Medical College and Hospital, Mysore, India. Instrumentation and Analytical Conditions A HPLC with the UV detector was used for this research work. Here the separation was done using Phenomenex C-18 column. The mobile phase was a mixture of phosphate Buffer (pH adjusted to 5 with potassium hydroxide) and acetonitrile (90:10) v/v. The mobile phase was filtered through 0.45 ÃŽ ¼ membrane filter before its use, degassed with a helium sparge for 15min at flow rate of 1.0 mL min-1. The column was maintained at room temperature 20 ±100C. The injection volume of samples was 10 ÃŽ ¼L. The analyte was monitored at wavelength of 230 nm and optimized chromatographic conditions are shown in Table-1. 2.3.Preparation of mobile phase: Phosphate buffer of pH 5 was prepared by dissolving 1.36 gm ofPotassiumdihydrogenorthophosphate in 1000 mL of water and it was sonicated for 5 minutes, then the pH was adjusted using potassium hydroxide solution. It was than filtered by vaccum filteration. Finally the mobile phase was prepared by mixing phosphate buffer and acetonitrile in the ratio 90:10v/v. 2.4.Preparation of standard and sample solution SeparatelyweighedquantityofCFXsodium(10mg)andSBMsodium (10mg)was transferred into a 100mL volumetricflaskandmadeupto100mLwithwatertoget100  µg mL-1 ofCFXsodiumand100  µg mL-1 ofSBM. From this, different solutions containing the mixture of CFXsodium(20-150  µg mL-1) and SBMsodium(10-75  µg mL-1) were prepared. For the preparation of sample solution, Cetriax-Spowder for injection(containing1gmof CFXand0.5gmof SBM)was transferred to a 100 mL volumetric flask. Distilled water was added, and then swirled to dissolve it, diluted to 100 mL with the same solvent. 2.5.Preparation of calibration curve: Five different concentrated solutions containing mixture of CFX (20-150  µg mL-1) and SBM (10-75  µg mL-1) were injected onto HPLC. A calibration curve was prepared taking concentrations on X-axis and Peak Area on Y-Axis. 2.6.Preparation of plasma samples: Plasma samples of CFX and SBM was prepared by the protein precipitation method. A blank was prepared by taking 0.1mL of rat plasma and to this 1.9 mL of acetonitrile was added and sample was prepared by taking 0.1 mL of combination of CFX and SBM (which were mixed in equal volumes) and 0.1 mL of rat plasma was added to the 2 mL Eppendorf tubes containing 1.8 mL of acetonitrile. These samples were centrifuged for 10 min at 10,000 rpm. The supernatant solution filtered through 0.45 µ syringe filter and transferred to HPLC vials. RESULTS AND DISCUSSION 3.1 Method Development Taking into consideration, the instability of CFX and SBM in strong alkaline and strong acidic condition, the pH value of the mobile phase should be limited within the range of 3à ¢Ã¢â€š ¬Ã‚ 7, since mild acidic pH favours the retention and separation of two drugs on Cà ¢Ã¢â€š ¬Ã‚ 18 column. After few trials, phosphate buffer with pH 5 was finalized. The method development started with the methanol and phosphate buffer as drugs did not elute in this mobile phase, so the organic phase was altered from methanol to acetonitrile. Both CFX and SBM in the mobile phase have no significant UV maximum, the wavelength of 230 nm was employed for the detection. After few trails Phenomenex C-18 column and binary mixture of phosphate buffer (pH 5) and acetonitrile (90:10 % v/v) was optimized as mobile phase which produced symmetric peak shape, good resolution and reasonable retention time for both the drugs (Table 1). The retention times of CFX and SBM for six repetitions were found to be 7.8  ± 0.02 min and 4.7  ± 0.006 respectively (Fig.2). (a) (b) Fig.2. LC chromatogram of rat blank plasma (a) plasma spiked with standard CFX and SBM(b) Table 1. Optimized chromatographic conditions Parameter Optimized condition Chromatograph HPLC with UV- detector Column C18 Column Mobile Phase Acetonitrile and pH-5 buffer in the ratio of 10:90(v/v) Flow rate 1.00 mL min-1 Detection 230nm Injection volume 10 ÃŽ ¼L Temperature column Room temperature 3.2.Method validation Validation is a process of establishing documented evidence, which offers a high degree of assurance that a specific activity will steadily yield anticipated result or product meeting its predetermined specifications and quality features [11]. The method was validated for different parameters like linearity, precision, recovery, accuracy, selectivity and sensitivity [12]. 3.2.1Selectivity Selectivity is defined as, the capability of an analytical method to distinguish and measure the analyte in the presence of other components in the sample [12]†. Selectivity is calculated by injecting extracted blank plasma and relating with the response of extracted LLOQ samples. Both the peaks of Ceftriaxone and Sulbactum did not interfere with any endogenous components. 3.2.2Sensitivity Sensitivity is measured using Lower Limit of Quantification (LLOQ). LLOQ is the lowest concentration of the standard curve that can be measured with acceptable accuracy and precision [12]†. The LLOQ was established using five samples independent of standards and determined the co-efficient of variation and appropriate confidence interval. 3.2.3.Linearity of Response To demonstrate the linearity of response, series of solutions ranging from (20-150  µg mL-1) of CFX and SBM of (10-75  µg mL-1) were prepared and injected onto the HPLC system following the described conditions. The graph was constructed between concentration vs. peak area and it was found that correlation co-efficient and regression analysis were within the limits and the results are summarized in the Table 2, and the calibration graphs are shown in Fig. 3 and Fig. 4 for CFX and SBM respectively. Fig.3. Calibration graph of CFX Fig.4. Calibration graph of SBM Table 2. Linearity of CFX and SBM Parameters CFX SBM Retention time (min) 7.3 4.6 Linear range (ppm) [n=6] ( µg mL-1) 20-150 10-75 Correlation coefficient (r2) 0.996 0.997 Slope 1513.1 155.58 Intercept 272333 61596 Lowest limit of quatification LLOQ ( µg mL-1) 0.87 0.96 3.2.4.Recovery â€Å"The recovery of an analyte is the detector response achieved from an quantity of the analyte added to and extracted from the biological matrix, correlated to the detector response found for the true concentration of the pure authentic standard†[12]. â€Å"Recovery of the analyte is not necessary be 100%† [12]. This experiments were performed by comparing the analytical results for extracted samples at three different concentrations (low, medium, and high) with unextracted standards that represent 100% recovery. Results are summarised in Table 3. Table 3. Recovery studies of CFX sodium and SBM Concentration of CFX and sulbactam Amount recovered% for CFX Amount recovered% for SBM Low 98.7% 99.9% Medium 96.8% 98.9% High 99.3% 98.6% 3.2.5.Accuracy and Precision For validation of this bioanalytical method, precision and accuracy should be determined using minimum of five determinations per concentration level (excluding blank samples). The mean value should be within  ± 15% of the theoretical value, except at LLOQ, where it must not differ by more than  ± 20%. The accuracy and precision around the mean value should not be beyond 15% of the CV except for LLOQ, where it should not exceed by 20% of the CV. The accuracy of the analytical method defines the closeness of agreement between the test value and the reference value. The precision of the analytical method describes the closeness of frequent individual measures of analyte. Accuracy is expressed in terms of % obtained. Precision is expressed in terms of coefficient of variation (CV). The statistical method for determination of the accuracy and precision should be predefined and calculated according standard practise. Accuracy and Precision should be demonstrated for the low, medium, high and LLOQ QC samples, within a single run and between different runs results are summarised in Table 4 5. % CV (precision) =100 x Standard deviation/Mean Table 4. Accuracy and Precision of CFX Theoretical concentration ( µg/mL) Measured concentration ( µg/mL) Intra-day Inter-day %CV Accuracy (%) %CV Accuracy (%) 20 0.98 98.4 1.42 96.1 100 0.76 103.7 1.32 102.3 150 1.34 99.5 1.7 98.7 Table 5. Accuracy and Precision of SLB Theoretical concentration  µg mL-1 Measured concentration ( µg mL-1) Intra-day Inter-day %CV Accuracy (%) %CV Accuracy (%) 10 0.96 101.7 0.76 95.6 50 1.00 99.8 1.2 103.4 75 1.02 97.3 1.04 97.4 3.2.6.Stability studies Freeze and Thaw Stability Stability of analyte was determined with three freeze and thaw cycles. All the three aliquots at low, medium and high concentrations were stored at the proposed storage temperature for 24 hours and defrosted unassisted at room temperature. When completely thawed, the samples were again frozen for 12 to 24 hours under the same conditions. The same cycle was repeated two more times, and then analyzed after the third cycle. Short-Term Temperature Stability Three aliquots of low, medium and high concentrations were thawed at room temperature and at this temperature sample was kept from 4 to 24 hours and analyzed. Long-Term Stability The storage time in a long-term stability assessment should surpass the time between the date of first sample collection and the date of last sample analysis. Long-term stability was determined by storing three aliquots of the low, medium and high concentrations under the same conditions as that of the study samples. The concentrations of all the stability samples were related to the mean of back-calculated values for the standards at the suitable concentrations from the first day of long-term stability testing. Stock Solution Stability The stability of stock solutions of drug was estimated at room temperature for 6 hours. After the desired storage time, the stability was confirmed by comparing the instrument response with that of newly prepared solutions Results are summarised in Table 6. Table 6. Stability studies of CFX and SBM Stability  µg mL-1 (error %) CFX  µg mL-1 (error %) SBM 20 100 150 10 50 75 Freeze-thaw 84.5 93.3 94.9 88.5 96.3 97.9 Long term 100.5 100.6 100.8 100.5 101.6 100.8 Short term 93.9 97.6 101.4 93.9 93.6 103.4 Stock Solution 95.6 97.6 93.2 95.3 96.8 98.5 SUMMARY In this work, a simple, stability indicating, accurate and validated stability indicating HPLC method for the simultaneous determination of ceftriaxone and sulbactam in their pharmaceutical formulation was developed. The method was validated according to FDA guidelines. CFX and SBM were eluted at 7.3 min and 4.6 min respectively. The correlation coefficient (r2) for CFX and SBM were found to be 0.996 and 0.9976 respectively. Lower Limit of quantification (LLOQ) was found to be 0.87  µg mL-1 for ceftriaxone and 0.96  µg mL-1 for sulbactam. The %CV for the intraday and interday precision were found to be CONCLUSION The method includes simple and precise method for simultaneous determination of CFX sodium and SBM. It produces symmetrical peak shape, good resolution and reasonable  retention time for both drugs. So this method can be appropriate for the   simultaneous estimation of CFX sodium and SBM in quality control studies for routine analysis. AKNOWLEDGMENT The authors are thankful to The Principal, JSS College of Pharmacy, JSS University, Mysore for providing all necessary facilities to carry out the research. The authors are also thankful to Strides Arco labs, Bangalore for providing the pure salbactum sodium and ceftriaxone sodium as gift samples. References Rang HP, Dale MM (1993). Pharmacology, (2nd ed.). Churchill Livingstone, ELBS. Physicians Desk Reference (1997). American Academy of Physician Assistants (51th ed). Patel FM, Dave JB , Patel CN, spectrophotometric methods for simultaneous estimation of cefuroxime sodium and sulbactam sodium in injecton, International journal of pharmaceutical sciences and research.2012; 3(9), 3513-3517. Jasmin Shah,Rasul Jan M,Sultan Shah,Naeem M, Spectrofluorimetric Protocol for Ceftriaxone in Commercial Formulation and Human Plasma After Condensation with Formaldehyde and Ethyl Acetoacetate, Journal of Fluorescence.2011; 21(6), 2155-2163. Shrivastava SM, Singh R, Ariq A, Siddiqui MR, Yadav J, Negi PS, Chaudhary M, A novel high performance liquid chromatographic method for simultaneous determination of ceftriaxone and sulbactam in sulbactomax, Inter Journal of Biomed Sci. 2009; 5(1), 37-43. Durairaj S, Annadurai T, Palani Kumar B, Arunkumar S, Simultaneous Estimation of Ceftriaxone Sodium and Sulbactam Sodium using Multi-Component Mode of Analysis, Inter Journal of ChemTech Research. 2010; 2(4), 2177-2181. Huang Ying, Liang Maozhi, Yu Qin, Jiang Lei,Shi Yingkang, Determination of ceftriaxone in human plasma by HPLC, Chinease Journal of Antibiotics.2000; 25,109. Zhao Xi, Zhang Dan, Chen Hong, Determination of cefotaxime/sulbactam in plasma and pharmacokinetics in dogs by high performance liquid chromatography. Zhongguo Kangshengsu Zazhi. 2004; 29(10): 614-616. Jelinek I, Krejcirova H, Dohan J, Roubal Z, Determination of sulbactam in human serum using capillary electrophoresis,Cesk Farm.1990; 39,305–307. Foulds G, Gans DJ, Girard D, Whall TJ, Assays of sulbactam in the presence of ampicillin.Therm Drug Monit.1986;2,223–237. Lloyd RS, Joseph JK, Joseph LG (1997). Practical HPLC Method Development (2ndedition) New York: Wiley-Blackwell. FDAUS. Guidance for Industry: Bioanalytical Method Validation. Rockville, MD, USA: US Department of Health and human services, US FDA/Center for Drug Evaluation and Research; 2001.

why kids kill parents Essay example -- essays research papers

Introduction to Psychology Why kids kill parents BY Kathleen M. Heide Psychology Today Magazine HOW A LEGACY OF CHILD ABUSE LEADS TO HOMICIDE I chose this article because I have always been astonished at how children can do this to their parents and what drove them to the choices they made. Did they have any other choices or did they use all of their chooses up that they had? This article supplied a lot of answers to me. According to this Article: Between 1977 and 1986, more than 300 parents were killed each year by their own children. &nb... ...ot his father once, he was then afraid his father would be okay and come after him again when he was well, so he shot him again to make sure his father would not be able to do that. To end with a quote from the article; " The true killer in these cases is child mistreatment." "Damage comes not only in human carnage but in the death of the human spirit that persistent abuse often carries out."

Motivation and Organizational Culture

3 Motivation and Organizational Culture Tawny Christensen HCA 250 March 14, 2013 Darlene Tomlinson Motivation and Organizational Culture Assignment In any given business or workplace environment the employees that work there should be the most valued assets. Research has shown that personnel who are happy and taken care of are much more productive than those who are not, therefore establishing a more pleasing work atmosphere. That being said, business leaders and managers alike play an extremely important role in workplace psychology.According to our text, leadership is the process of leading a group and influencing its members to achieve common goals. All managers are leaders (Robbins, DeCenzo, and Coulter, 2011). In today’s society more managers are leading through empowerment by allowing team members to be involved in the decision making process. More and more employees â€Å"are developing budgets, scheduling workloads, controlling inventories, solving quality problems, a nd engaging in similar activities that until very recently were viewed exclusively as part of the manager’s job,† (Robbins, DeCenzo, and Coulter, 2011).As mentioned earlier, research has shown that employees who are pleased are far more beneficial than employees who are not. There are many things that managers can do to ensure that their employees feel valued, and remain satisfied. First of all, it is important that managers avoid using intimidation. To intimidate means to make nervous, or instill fear. In some cases an individual can take advantage of their title and turn in to an authoritarian. In a sense this means that the big guys squash the little guys. Employees

Remembering an Artist essays

Remembering an Artist essays Ron Jeremy, Vincent Van Gogh, Emily Carr, Madonna and Miles Davis are all considered artists in their own right. The lives and achievements of these men and women are common public knowledge because theyre taught at school. If theyre not taught from a teacher as part of the curriculum then theyre learned by socializing with friends at school. This creates the misconception amongst young people that; artists who dont fall under this category just arent good. I would like to present one of these artists who arent good. Georgia OKeeffes story isnt one that involves a messed up childhood. She never had to fight off a drug addiction, she was never insane, and her artwork was never made through the inspiration of insanity. She was, however, a gifted child. Relatively speaking, Georgia OKeeffe had a normal childhood. Early in her life, the theories and teachings of her mentors heavily influenced her paintings. Her art therefore, was a unique blend of abstract and representational. She was k nown for the purity, boldness, and clarity of her still-life compositions. After the death of her husband, she moved to New Mexico, there her paintings began to reflect the daily life of the culture there. The very land she lived on became her inspiration. Although Georgia OKeeffe is not very popular with the new generation, she is very much worth remembering. Unlike most other women in the early 20th century, Georgia OKeeffe did not let discrimination ruin her life. She used her art to capture the worlds attention and also used it as a way to shield herself from societys problems. Born in Sun Prairie, Wisconsin in 1887, Georgia O'Keeffe was the second of seven children. When she a little girl, she took art lessons. Her teachers thought she was very gifted and encouraged her to paint more. After she graduated from high school, she wanted to become a...

Education Term Paper

Education Term Paper Education Term Paper Education Term Paper: Pedagogy Devise. With money in your pocket you are wise, handsome and sing well too, - said one well-known celebrity. Really, everyone could be educated (get higher education), but not well-breaded. You could get a diploma but not knowledge. Think about it... Your education term paper is your face, your thoughts and your intelligence. They should be individual, unique, and also match your inner world. Education Term Paper: The Point. Education may contain a lot of aspects. It includes learning a great number of disciplines, different kinds of applied and humanistic science. During the academic year you should pass your education term papers. Education is directly connected with knowledge handover and bringing up a new generation. In different educational institutions educators direct the education process of students; also they may manage a variety of subjects, for example reading, physics, writing, philosophy, science and history. After you gad reach to the end of the course you should be checked by writing education term papers. Education Term Paper: Education For Children (Students) Today when you have already left school, you are not a pupil anymore, you are an applicant. So, you have a splendid opportunity to choose and enter any college you like. Youth is a discovery time. You should think over and make your decision. If you keen on technique you may continue your studying in Technique College. However, if you do not interested in machines and engineering, moreover you belong to the people who prefer live communication, which directly connected with finding yourself among people, you should choose institute specializing in philology and pedagogy In that case passing a education term papers would be a pleasure for you. Education Term Paper: Education For Adults (Teachers) For a long time teachers have been educated and respectable people. Teachers were given the knowledge and educate children. They take a great effort to the activity they do. Teaching is the style of life. Teacher gives a lot and gets a lot. They give knowledge and upbringing positive qualities, and in the same way they get a pleasant communication among children and a lot of work to do, in particular checking education term papers.

Public Health infrastructure Research Paper Example | Topics and Well Written Essays - 500 words

Public Health infrastructure - Research Paper Example No single effort toward the infrastructural development can undermine the factors which intensify the virus spread. This comprehensive approach should be supported by adequate funding, workforce, and laboratory facilities, especially where the disease is highly endemic. However, having a well praised health infrastructure is not what all needed, but it must be supported with available scientific evidence for decision making. Creating on time administration policies is also important. These policies must include prevention programs and other aspects regarding the operational research. The research process in the public health infrastructure must be more clear, specific, and effective than they are in the current scenario. The research team should conduct significant research missions regarding the means to countermand the spread of HIV/AIDS. They should adopt programs from the international research leaders like the MHRP. The U.S. Military HIV Research Program (MHRP), 1986, protected the service personnel as well as the global community by diminishing the risk of HIV infection (Uganda). Until now, MHRP has been leading the International HIV vaccine development efforts, having five research sites in Asia and Africa.

Woolley v. Hoffmann-La Roche, Inc. and Lucy v. Zehmer Essay

Woolley v. Hoffmann-La Roche, Inc. and Lucy v. Zehmer - Essay Example In that case, the court is changing the employment law in Jersey State. In such a case when the plaintiff is not available, it is impossible to continue with the case, and automatic cessation of the case occurs. Any death occurring before the case is over and the jury have made the decision leads to final disqualification as one party that had information on the case is no longer available. The court is in the process of changing the court analysis in the country. The opinions of the employer are fair. Basic honesty is necessary in employment contracts. The jury had to make tight decisions since it is dependable on the future judgments. The common law of the country should hence be promoted for the sake of justice (Werhane, Radin, Bowie, & Wiley, 2004). For the employer to avoid such future problem, he should ensure that the employment manual is different from the employment contract to make everything clear. The whole task of selling the land ends up in a total dispute. It is accompanied by corruption such as bribing for the sake of convincing the buyer to fix the bargain. In addition to that, it seems that Zehmer was intoxicated with â€Å"drinks â€Å"at the time of the sales process, later confirming that the whole process was a joke. In addition to that, the price for the quoted land is far much low according to the economic standards, such that one may see it a joke buying such huge piece at such a low price. Zehmer also makes a commitment to sell the farm without making a prior and sober agreement with the wife but simply seduces her to sign the offer. If the defendants were in a position to accept that the whole process was a foul and joke, it would have implications or any change. It would remain a breach of contract of sale between the two parties. When one is making an agreement to sell a property or for employment purposes, he should note that, it remains critical to submit to the terms of the offer and to oblige to it in full. In the